Building 600, Room 381 (920 square feet)
Our laboratory, located in room 384 of the old dental school building, is equipped for cellular, biochemical and molecular biology protocols for histochemistry, immunohistochemistry and in situ hybridization of sectioned tissues and the isolation and characterization of proteins and nucleic acids by electrophoresis, hybridization techniques and quantitative RT-PCR
The Craniofacial Biology Research Laboratory is studying growth, development and function of the masticatory system and the contribution of intrinsic laryngeal muscles to the quality of human voice. These cranial muscle groups have been found to express novel or unusual combinations of myosin heavy chain motor proteins which produce distinctive physiological properties when tested at the single fiber level. Cellular and molecular analyses of fiber types in masticatory and laryngeal muscles of human subjects indicate that fiber-type properties associate, respectively, with 1). development of jaw dentofacial deformations and malocclusion and 2). variations in vocal fold function. We are now investigating whether expression levels and genotypic polymorphisms among masticatory muscle genes may associate with fiber type composition and malocclusion in a large group of human subjects. For voice experiments, being done in conjunction with Dr. Carrie Tellis at Misericordia University, we are using a newly developed recording device to measure pre and post exercise oxygen consumption and hemoglobin levels in the human thyroarytenoid muscle and comparing these data to fiber type properties for calibration of muscle fatiguability and pathology in vocal disorders.
Vecchione L, Byron C, Cooper GM, Barbano T, Hamrick, Sciote JJ, Mooney MP. Craniofacial morphology in myostatin-deficient mice. J Dent Res 2007;11:1068-1072.
Horton MJ, Sciote J. Quantification of myosin heavy chain RNA in human laryngeal muscles: Differential expression in the vertical and horizontal posterior cricoarytenoid muscle. Laryngol 2008; 118:472-477.
Vecchione L, Byron C, Cooper GM, Barbano T, Hamrick MW, Sciote JJ, Mooney MP. Age related changes in craniofacial morphology in GDF-8 (myostatin) deficient mice. Anat Rec 2010; 293:32-41.
Tellis, C.M., Sciote, J.J., Rosen, C.A., Taylor, T.T., Yaruss, J.S., Verdolini K. A histochemical analysis of mitochondrial abnormalities in type I fibers of human posterior cricoarytenoid muscle. J Voice 2011; in press.
Raouel J, Rowlerson A, Stevens L, Maurage C-A, Duhamel A, Sciote J, Ferri J. Human masticatory muscle phenotype varies with mandibular asymmetry. J Craniofac Surg. 2011;May issue:accepted.
Cray J, Vecchone L, Byron C, Cooper G, Sciote J, Siegel M, Mooney MP. Masticatory hyper-muscularity is not related to reduced cranial volume in myostatin-knockout mice. J Anat 2011;submitted.
Sciote JJ, Horton MJ Rowlerson AM, Ferri J, Close J, Raoul G. Human masseter, malocclusions and muscle growth factor expression. J Oral Maxillofac Surg 2011; submitted.
Tassopoulou M, Deeley K, Harvey E, Sciote J, Vieira AR. Genetic variation in Myosin 1H contributes to mandibular prognathism. Am J Orthod Dentofac Orthop 2011:submitted.