The Craniofacial Biology Research Laboratory is equipped for cellular, biochemical and molecular biology protocols for histochemistry, immunohistochemistry and in situ hybridization of sectioned tissues and the isolation and characterization of proteins and nucleic acids by electrophoresis, hybridization techniques and quantitative RT-PCR.

Craniofacial Biology Research Laboratory

Director

James Sciote, DDS, MS, PhD
Professor, Department of Orthodontics
Phone: 215-707-4620
e-mail: jjs6@dental.temple.edu

Facilities

Building 600, Room 381 (920 square feet)

Our laboratory, located in room 384 of the old dental school building, is equipped for cellular, biochemical and molecular biology protocols for histochemistry, immunohistochemistry and in situ hybridization of sectioned tissues and the isolation and characterization of proteins and nucleic acids by electrophoresis, hybridization techniques and quantitative RT-PCR

Research Focus

The Craniofacial Biology Research Laboratory is studying growth, development and function of the masticatory system and the contribution of intrinsic laryngeal muscles to the quality of human voice. These cranial muscle groups have been found to express novel or unusual combinations of myosin heavy chain motor proteins which produce distinctive physiological properties when tested at the single fiber level. Cellular and molecular analyses of fiber types in masticatory and laryngeal muscles of human subjects indicate that fiber-type properties associate, respectively, with 1). development of jaw dentofacial deformations and malocclusion and 2). variations in vocal fold function. We are now investigating whether expression levels and genotypic polymorphisms among masticatory muscle genes may associate with fiber type composition and malocclusion in a large group of human subjects. For voice experiments, being done in conjunction with Dr. Carrie Tellis at Misericordia University, we are using a newly developed recording device to measure pre and post exercise oxygen consumption and hemoglobin levels in the human thyroarytenoid muscle and comparing these data to fiber type properties for calibration of muscle fatiguability and pathology in vocal disorders.

Faculty

Dr. James Sciote, Professor

Staff

Dr. Michael Horton, Assistant Research Professor

Representative Publications

Vecchione L, Byron C, Cooper GM, Barbano T, Hamrick, Sciote JJ, Mooney MP. Craniofacial morphology in myostatin-deficient mice. J Dent Res 2007;11:1068-1072.

Horton MJ, Sciote J. Quantification of myosin heavy chain RNA in human laryngeal muscles: Differential expression in the vertical and horizontal posterior cricoarytenoid muscle. Laryngol 2008; 118:472-477.

Vecchione L, Byron C, Cooper GM, Barbano T, Hamrick MW, Sciote JJ, Mooney MP. Age related changes in craniofacial morphology in GDF-8 (myostatin) deficient mice. Anat Rec 2010; 293:32-41.

Tellis, C.M., Sciote, J.J., Rosen, C.A., Taylor, T.T., Yaruss, J.S., Verdolini K. A histochemical analysis of mitochondrial abnormalities in type I fibers of human posterior cricoarytenoid muscle. J Voice 2011; in press.

Raouel J, Rowlerson A, Stevens L, Maurage C-A, Duhamel A, Sciote J, Ferri J. Human masticatory muscle phenotype varies with mandibular asymmetry. J Craniofac Surg. 2011;May issue:accepted.

Cray J, Vecchone L, Byron C, Cooper G, Sciote J, Siegel M, Mooney MP. Masticatory hyper-muscularity is not related to reduced cranial volume in myostatin-knockout mice. J Anat 2011;submitted.

Sciote JJ, Horton MJ Rowlerson AM, Ferri J, Close J, Raoul G. Human masseter, malocclusions and muscle growth factor expression. J Oral Maxillofac Surg 2011; submitted.

Tassopoulou M, Deeley K, Harvey E, Sciote J, Vieira AR. Genetic variation in Myosin 1H contributes to mandibular prognathism. Am J Orthod Dentofac Orthop 2012;141:51-59. PMC3600843

Nicot R, Hottenstein M, Raoul G, Ferri J, Horton M, Tobais J, Barton E, Sciote JJ. Asymmetry pathway genes are down regulated in facial asymmetry. J Craniofac Surg 2014;25(6):e548-555. PMC4224967

Zebrick B, Nicot R, Raoul G, Ferri J, Horton M, Vieira A, Sciote JJ. ACTN3 R577X genotypes associate with Class II and deep bite malocclusions. Am J Orthod Dentofac Orthop;2014;146(5):603-611. PMC4254725

Nicot R, Vieira AR, Raoul G, Delmotte C, Ferri J, Sciote JJ. ENPP1 and ESR1 genotypes influence temporomandibular disorders development and surgical-treatment response in dento-deformities. J Craniomaxillofac Surg;2016;44:1226-1237. PMC5026604

Constant M, Nicot R, Vieira AR, Raoul G, Sciote JJ, Ferri J. Condylar geometry variation is associated with ENPP1 variant in a population of patients with dento-facial deformities. J Cranio-Maxillo-Fac Surg;2017;45(6):826-836.

Chung K, Richards T, Nicot R, Vieira A, Raoul G, Ferri J, Sciote JJ. ENPP1 and ESR1 genotypes associated with subclassifications of craniofacial asymmetry and severity of TMD. Am J Orthod Dentofac Orthop; November 2017:;152(5):631-645.

Sahin T, Garreau E, Komakli Y, Sciote JJ, Ferri J. Mandibular anterior segmental subapical osteotomy for incisor axis correction. J Stomatol Oral Maxillofac Surg;2017:online 19 June, 2017.