Regenerative Health Research Laboratory Cover Photo

Regenerative Health Research Laboratory

Director

Maobin Yang, DMD, MDS, PhD
Professor, Department of Endodontology
Phone: 215-707-5789
e-mail: myang@temple.edu

Facilities

Building 600, Room 403 (860 square feet)

The laboratory is categorized as a biosafety level 2 (BSL2). It houses necessary equipment for cellular and molecular biomedical research, including a biosafety culture hood, two CO2 incubators, an inverted fluorescent phase contrast microscope and a phase contrast microscope. The laboratory also equipped with an ultra-low temperature freezer and refrigerator, cryogenic storage vessel for cell storage, centrifuges, chemical fume hood, milipore water purification system, spectrophotometer, real time PCR machine, Western Blot processing system, FluorChem M image system, 810-nm GaAlAs laser machine and other basic lab research equipment and instruments.

Research Focus

The regenerative health research laboratory focuses on developing cutting-edge technologies in the field of regenerative dentistry, especially in the regenerative endodontics and periodontics. The successful regeneration of dental tissues needs all components of the tissue engineering triad: stem cells, growth factors, and scaffolds. Our laboratory has successfully generated a biomimetic scaffolds that support the regeneration of pulp-dentin complex and periodontal ligament. Our studies also study different types of stem cells from human oral tissue, including stem cells from apical papilla (SCAPs), dental pulp stem cells (DPSCs), dental follicle stem cells (DFSCs), and periodontal ligament stem cells (PDLSCs).

In addition, we are studying the Low-level Laser Therapy (LLLT) and its applications in the field of regenerative endodontics. LLLT is believed to have photobiomodulation effect on stem cells. We are using “cold laser”, the low level laser between the wavelengths 632-1064 nm and has no thermal effect on the tissues, to study its effect on dental stem cell differentiation.

The lab provides facilitates and research trainings for the post-baccalaureate students, dental students, postgraduate students, as well as the PhD students from main campus, and the international visiting students and scholars.

Faculty

Dr. Maobin Yang, Assistant Professor

Dr. Jong Kim, Associate Professor

Staff

Dr. Dalia Alzebdeh, Postdoctoral Research Associate

Collaborators

Dr. Peter I. Lelkes, Chair and Professor
Department of Bioengineering, College of Engineering, Temple University

Dr. Junqi Ling, Dean and Professor
Guanghua School of Stomatololgy, Sun Yat-sen Universtiy

Representative Publications

  1. Zeng Q, Nguyen S, Zhang H, Chebrolu H, Alzebdeh D, Badi B, Kim J, Yang M. Release of Growth Factors into Root Canal by Irrigations in Regenerative Endodontics. Journal of Endodontics, 2016, 11
  2. Verma P, Nosrat A, Kim JR, Price JB, Wang P, Bair E, Xu HH, Fouad AF. Effect of Residual Bacteria on the Outcome of Pulp Regeneration In Vivo. J Dent Res. 2016 Sep 30.
  3. Kim J, Nosrat A, Fouad AF. Interfacial characteristics of Biodentine and MTA with dentin in simulated body fluid. J Dent 2015 43(2):241-247.
  4. Maobin Yang, Junqi Ling, Xi Wei, and Qian Zeng. Chapter 13. Craniofacial tissue reconstruction with mesenchymal stem cells derived from dental tissue and bone marrow. Nanomaterials and regenerative medicine. IAPC publishing. January 2016.
  5. Maobin Yang, Qian Zeng, Maria Pereira Vieira and Bernardo Olsson. Chapter 2. Three-dimensional Printing in Dentistry: An Advanced Technology for Craniofacial Regeneration. Mesenchymal Stem Cells and Craniofacial Regeneration. Benthamscience. 2016
  6. Gonzalez R, Zeng Q, Nissan R, Yesilsoy C, Yang M. Dentin Hypersensitivity Related to Apical Fenestration: A Case Report. Austin Journal of Dentistry, 2016, 3(4).
  7. Yang M, Zeng Q, Regenerative Endodontics: A New Treatment Modality for Pulp Regeneration.  International Journal of Stomatology, 2016, 9.
  8. Yang M, Hongming Zhang, Riddhi Gangolli. Advances of Mesenchymal Stem Cells Derived from Bone Marrow and Dental Tissue in Craniofacial Tissue Engineering. Current Stem Cell Research and Therapy, 2014 (9): 150-161.
  9. Nosrat A, Kim J, Verma P, Chand P. Tissue Engineering Considerations in Dental Pulp Regeneration. Iran Endod J 2014 9(1):30-40.
  10. Yang M.  Regenerative Endodontics: A New Treatment Modality for Pulp Regeneration. JSM Dent 2013:1: 1011-1012.
  11. Yang M, Trettel LB, Adams DJ, Harrison JR, Canalis E, Kream BE. Col3.6-HSD2 transgenic mice: a glucocorticoid loss-of-function model spanning early and late osteoblast differentiation. Bone, 2010 Sep; 47(3): 573-82.
  12. Kim J, Uchiyama T, Carrilho M, Agee AK, Mazzoni A, Breschi L, Carvalho RM, Tjäderhane L, Looney S, Wimmer C, Tay FR, Pashley DH. Chlorhexidine binding to mineralized versus demineralized dentin powder. Dent Mater 2010 26(8):771-778.
  13. Kim J, Arola DD, Gu L, Kim YK, Mai S, Liu Y, Pashley DH, Tay FR. Functional biomimetic analogs help remineralize apatite-depleted demineralized resin-infiltrated dentin via a bottom-up approach. Acta Biomaterialia 2010 6(7):2740-2750.
  14. Kim J, Gu L, Breschi L, Tjäderhane L, Choi KK, Pashley DH, Tay FR. Implication of ethanol wet bonding in hybrid layer remineralization. J Dent Res 2010 89(6):575-580.
  15. Kim J, Mai S, Carrilho MR, Yiu CK, Pashley DH, Tay FR. An all-in-one adhesive does not etch beyond hybrid layers. J Dent Res 2010 89(5):482-487.
  16. Kim J, Vaughn RM, Gu L, Rockman RA, Arola DD, Schafer TE, Choi KK, Pashley DH, Tay FR. Imperfect hybrid layers created by an aggressive one-step self-etch adhesive in primary dentin are amendable to biomimetic remineralization. J Biomed Mater Res Part A 2010 93(4):1225-1234.
  17. Kim YK, Yiu CK, Kim JR, Gu L, Kim SK, Weller RN, Pashley DH, Tay FR. Failure of a glass ionomer to remineralize apatite-depleted dentin. J Dent Res 2010 89(3):230-235.
  18. Yang M, Kream BE. Calcitonin induces expression of the inducible cAMP early repressor in osteoclasts. Endocrine 2008, Jun;33(3): 245-53.