Our research facilities consist of two interdependent laboratories. The first contains several service components that are needed to support the research activities in the second or main laboratory area. These components include a walk-in warm room/ incubator, a walk-in cold room/ refrigerator, a water purification system, a laboratory scale autoclave, equipment for processing glassware, and an instrument room. The instrument room contains such large pieces of laboratory equipment as: an ultracentrifuge, a superspeed centrifuge, ultracold freezers, refrigerators, a lyophilizer (freeze-drying apparatus), and liquid scintillation counters (radioactivity monitors). In addition, this laboratory contains a fully equipped tissue culture facility.
The second laboratory contains large areas of bench space, cabinets, and storage shelves. This work area contains all the bench-top (smaller) pieces of laboratory equipment, such as balances, electrophoresis apparatus, thermal cyclers (for polymerase chain reactions), micro centrifuges, water baths, etc. A few frequently used large pieces of equipment, such as an ice maker, a second high speed centrifuge, a second ultracold freezer, a conventional refrigerator, etc. are housed here. Attached to the second laboratory is a darkroom with equipment for auto radiography processing, fluorescent photography and photocopying.
Another area in this laboratory has been designed as a sterile room for work requiring critical aseptic technique. Finally, office space for several investigators is also provided in this laboratory. Altogether, this facility totals more than 2,200 square feet of space.
Dr. Roy Stevens was the recipient of an NIH/NIDCR research grant. He was one of only three recipients nationwide to be awarded an American Recovery and Reinvestment Act-funded NIH/NIDCR research grant in 2010. This is Dr. Stevens’ second NIH grant since becoming a Temple faculty member, and makes him the only Temple University Kornberg School of Dentistry faculty member to be awarded an NIH grant in the past 20 years. The $420,366 grant will be used to investigate the “Therapeutic potential of Enterococcus faecalis bacteriophage phiEf11”.
Current Research Projects
Several exciting research projects are ongoing in our research facility. These include:
-Investigation of virulence factors of Enterococcus faecalis.
-Isolation of a bacterial virus induced from lysogenic strains of Enterococcus faecalis recovered from infected root canals.
-Characterization of the genome of the Enterococcus faecalis bacteriophage.
-Testing the ability of lasers to disinfect dentin.
-Evaluation of the mechanical properties and machining capabilities of a new series of Endodontic files.
-Screening of patient sera for the presence of antibodies to bacteriophage ØAa.
-Comparison of gene expression in lysogenic and nonlysogenic strains of Actinobacillus actinomycetemcomitans.
-Investigation of the mechanism of control of lysogeny in Actinobacillus actinomycetemcomitans
-Investigation of the mechanism of fibroblast-mediated phage induction in lysogenic strains of Actinobacillus actinomycetemcomitans
-Investigation of the potential application of a Dental Fluoroscopic imaging system.
-Comparison between medical fluoroscopy and intraoral radiography in terms of dose rate.
-Evaluation of the efficacy of an improved cervical plexus intraoral anesthetic technique.
-Effect of vehicle on the antibacterial activity of an iodine-containing calcium hydroxide intracanal medication